FAIRLEIGH DICKINSON UNIVERSITY

SCHOOL OF NATURAL SIENCE

FALL 2001

BIOL. 1125/1126 MICROBIOLOGY FOR THE HEALTH SCIENCE

4 Credits

Lecture 2 hours; Laboratory 4 hours

Course Description:

An introduction to the microbial world with emphasis on aspects of immunology, bacteriology, virology, mycology and parasitology which are relevant to the role of microorganisms in disease processes. The Laboratory will deal with the isolation and identification of common pathogenic and nonpathogenic organisms utilizing techniques of staining, culturing, fermentation reactions and microscopic inspection. This course is suitable for students planning a career in the health sciences and for liberal arts students with an interest in medical microbiology.

Instructor: Dr. Alice Benzecry
Office: Dickinson Hall, Room 4416
Phone # 692-2385 / E-mail: benzecry@fdu.edu
Office Hours: Mondays 2:00 – 3:00PM, Wednesdays 12:30 – 3:00PM
 

Lab. Instructor: Mr. Nick Agostino
Office: Dickinson Hall, Room 5535
Phone # 692-2240 / E-mail nick@fdu.edu

Requirement:

Jensen, Wright and Robson; Prentice Hall, Upper Saddle River, NJ 07458


BIOL. 1125 MICROBIOLOGY FOR THE HEALTH SCIENCE

LECTURE OUTLINE

WEEK
TOPIC
Assigned Text Reading
1
 History of Microbiology Chapter 1
2
 Survey of Microbial life

Microscopy

 Chapter 2

Chapter 3 
3
 Cellular Anatomy –Prokaryotic & Eukaryotic cells Chapter 4
4
 Microbial Metabolism –Metabolic functions Chapter 5
5
 Genetic Control of Cellular Functions Chapters 6 
EXAM 1
 Chapters 1-6
6
 Microbial Growth & Nutrition  Chapter 7
7
 Control of Microbial Growth –Sterilization &Disinfections

Chemotherapeutic Agents

 Chapter 8

Chapter 9
8
 Host-Microorganism Interactions Chapter 13
9
 Host Defense Mechanism

Immune Response 

 Chapters

10, 11, & 12
10
11
EXAM 2
 Chapters 7-13
Major Genera and species of Medically significant Bacteria, Pathogenesis and Clinical Diseases. Selective reading Chapters 14,15,

16,17,18,19,20,

21,22,23,24,25,

26 & 27
12
 Major Genera and species of Medically significant Bacteria, Pathogenesis and Clinical Diseases.
13
 Viruses and Viral Diseases Selective reading Chapters 28,29,

30,31,32,33,34,

35,36,37, & 38
14
 Eukaryotic Microbes and Parasites – Fungi & Protozoa Chapters 39-40
15
FINAL EXAM
  

  BIOL. 1126 MICROBIOLOGY FOR THE HEALTH SCIENCE


LABORATORY OUTLINE


 
Date
LAB
Topic
Assigned Reading
9/7  1 Introduction Laboratory safety and Protocol.  
9/10  2 Microscopy Ex. # 1 
9/14 3 Culturing of Microorganisms Ex. # 2
9/17 4 Examination of living microbes Ex. # 3
9/21 5 Staining: -Simple Stains  Ex. # 4 
9/24 6 Structural Stains Ex. # 7
9/28 7 Gram Stain Ex. # 5
10/1 8 Acid Fast Stain Ex. # 6 
10/5 9 Microbe culturing: Broth and Agar cultures Ex. # 8
10/8 10 Streak plate, Pure Culture Technique Ex # 9
10/12 11 -Pour Plate; Subculture Technique Ex.# 10
10/15 12 -Culturing Microorganisms from the Environment Ex. # 11
10/19 13 Destruction of Microorganisms- Moist and Dry Heat Ex. # 12
10/ 22 14 Autoclave / UV Radiation Ex. # 13
10/26 15 Disinfectant and Susceptibility Test Ex. # 14/15
10/29 16
11/2 17
MIDTERM EXAM ( Labs 1-16)
  
11/5 18 Nutritional and Physical requirements:

Selective and Differential Media 

 Ex. # 16, 19, & 20
11/9 19
11/12 20 Oxygen and Temperature Ex. # 28
11/16 21 Biochemical Tests: Carbohydrate Fermentation, Litmus Milk Reaction, Hydrolysis: Starch, Lipid, Casei, Gelatin. Ex.# 17
11/19 22
11/20 - 11/25 Thanksgiving Break
11/26 23 Nitrate Reduction, IMViC tests, Urease, Catalase, and Oxidase Test Ex. # 17 & 18
11/30 24 Unknown ID   
12/3  25 Unknown ID / Enterobacteriaceae Ex. # 24
2/10 26 Unknown ID  
12/7 27 Unknown ID  
12/14 28
FINAL EXAM
  

Attendance: Students are required to attend all lecture and laboratory sections. Lateness or absence of greater than 10% is considered excessive and could result in a lowering of your course grade.

GRADING CRITERIA:
 
Lecture………………….……….60%  (Three lecture exams 20% each)
Project…………………………..10%  Written report + Lab unknown.

( The instructor will discuss the characteristics to be evaluated)
 
Laboratory………………30% 10% Midterm exam
  10% Final Exam
  10% Lab performance + notebook

Lab performance: Competence in performing laboratory procedures, adherence to safety measures, and interpreting experimental results will be considered as part of your laboratory grade. Your instructor will base the evaluation on the following objectives.
  1. Follow all safety rules stated in the laboratory manual.
  2. Demonstrate a working knowledge of the care and use of the microscope.
  3. Use aseptic technique when handling cultures.
  4. Demonstrate evidence of preliminary preparation and organization when performing laboratory techniques.
  5. Perform selected laboratory techniques safe and correctly.
  6. Interpret laboratory results correctly.
Final letter grade will be assigned accordant to the following grade scale.
 
A+ 98-100
A 93-97
 A- 90-92
B+ 87-89
B 83-86
 B- 80-82
C+ 77-79
C 73-76
C-  70-72
D+ 67-69
D 62-66
 D- 59-61
F 0-58

Examination make-up policies:
All make-ups will only be administered the day of your final exam.

College regulations regarding cheating will be strictly enforced.

Note: Please bring a # 2 pencil and eraser to all exams.