CHEM 3281 – BIOCHEMISTRY

Instructor: Dr. Mihaela Leonida

Text: R. H. Garrett and C. M. Grisham,

Principles of Biochemistry with a Human Focus, Harcourt College Publishers, 2002.

Week	Topic
1	- Cells - Biomolecules and biological macromolecules - Water: the medium of life - pH - The Henderson-Hasselbalch equation - Titration curves - Amino acids
2	- Reactions of amino acids. The peptide bond - Chirality of amino acids - The primary sequence of amino acids - Experimental methods for protein sequencing - Secondary structure of proteins
3	- Protein folding - Tertiary and quaternary structure - Kinetics of enzyme-catalyzed reactions - Enzyme Inhibition
4	- Mechanisms of enzyme action - Regulation of enzyme activity - Review for exam I
5	- Exam I - Thermodynamics of biological systems - ATP – an intermediate energy-shuttle molecule
6	- Carbohydrates - Monosaccharides. Oligosaccharides. - Polysaccharides and their functions - Glycolysis
7	- Gluconeogenesis - TCA cycle
8	- Electron transport - Complexes I, II, III, and IVh - Oxidative phosphorylation - Review for exam II
9	- Photosynthesis
10	- Biological membranes - Fluid mosaic model - Transport across membranes
11	- Lipid metabolism - b oxidation - Fatty acid biosynthesis
12	- Amino acid metabolism - Integration of metabolism and hormone action - Structure of nucleic acids and nucleoproteins
13	- DNA - Information transfer: replication, recombination and repair
14	- RNA - Protein synthesis - Review for the final exam