Neena Philips, PhD

Scholarship and Teaching

(October 2009)

I. Publications

Original Papers

[1] Bashey RI, Philips N, Insigna F, Jimenez SA. (1993) Increased collagen

synthesis and increased content of type VI collagen in myocardium of tight skin mice. Cardiovascular Res 27:1061-1065.

[2] Philips N, Bashey RI, Jimenez SA. (1994) Collagen and fibronectin expression by cardiac fibroblasts from hypertensive rats. Cardiovascular Res 28: 1341-1347.

[3] Philips N, Bashey R, Jimenez SA. (1995) Increased a1 (I) procollagen gene expression in tight skin (TSK) mice myocardial fibroblasts is due to the absence of the strong inhibitory effect of a negative regulatory sequence that interacts with AP-1 transcription factor. J Biol Chem 270: 9313-9321.

[4] Philips N, Onwubalili N. (2002) Anti transforming growth factor-beta (TGF-b) increases the expressions of matrix metalloproteinase-1 (MMP-1) and growth factors in a renal adenocarcinoma cell line. Bios 73: 86-90.

[5] Philips N, Smith J, Keller T, Gonzalez S. (2003) Predominant effects of Polypodium leucotomos on membrane integrity, lipid peroxidation, and expression of elastin and matrixmetalloproteinase-1 in ultraviolet radiation exposed fibroblasts, and keratinocytes. J Dermatol Sci 32: 1-9.

[6] Philips N. (2003) An anti TGF-b increased the expression of transforming growth factor-b, matrix metallproteinase-1, and elastin, and its effects were antagonized by ultraviolet radiation in epidermal keratinocytes. J Dermatol Sci 33: 177-179.

[7] Philips N, Devaney J. (2003) Beneficial Regulation of Type I Collagen and Matrixmetalloproteinase –1 Expression by Estrogen, Progesterone, and its Combination in Skin Fibroblasts. J Am Aging Association 26: 59-62.

[8] Philips N, Keller T, Gonzalez S. (2004) TGF b like regulation of matrix mettalloproteinases by anti transforming growth factor-b and anti transforming growth factor-b1 antibodies in dermal fibroblasts: implications to wound healing. Wound Rep Reg 12: 53-59.

[9] Philips N, McFadden K. (2004) Inhibition of transforming growth factor-beta and matrix metalloproteinases by estrogen, and prolactin in breast cancer cells. Cancer Letters 206: 63-68.

[10] Philips N, Burchill D, O’Donoghue D, Keller T, Gonzalez S. (2004) Identification of benzene metabolites in dermal fibroblasts: Regulation of cell viability, apoptosis, lipid peroxidation, and expression of MMP-1 and elastin by benzene metabolites. Skin Pharmacol and Physiol 17: 147-152.

[11] Philips N, Keller T, Holmes C. (2007) Reciprocal Effects of Ascorbate on Cancer Cell Growth and the Expression of Matrix Metalloproteinases and Transforming Growth Factor-beta. Cancer Letters 256: 49-55.

[12] Philips N, Keller T, Hendrix C, Hamilton S, Arena R, Tuason M, Gonzalez S. (2007) Regulation of the Extracellular Matrix Remodeling by Lutein in Dermal Fibroblasts, Melanoma cells, and Ultraviolet Radiation Exposed Fibroblasts. Arch Dermatol Res 299: 373-379.

[13] Astner S, Wu A, Chen J, Philips N, Rius-Diaz F, Parrado C, Mihm MC, Goukassian DA, Pathak MA, González, S. (2007) Dietary Lutein/Zeaxanthin Reduces Photoaging and Photocarcinogenesis in Chronically UVB Irradiated SKH -1 Hairless Mice. Skin Pharmacol and Physiol 20: 283-281.

[14] Brieva A, Philips N, Tejedor R, Guerrero A, Pivel JP, Alonso-Lebrero JL, Gonzalez S. (2008) Molecular Basis for the Regenertive Properties of the Mollusk Criptomphalus aspersa. Skin Pharmacol and Physiol 21:15-22.

[15] Philips N, Arena R, Yarlagadda S. (2009) Inhibition of Ultraviolet Radiation mediated Extracellular Matrix Remodeling in Fibroblasts by Transforming Growth Factor-b. Bios: 80: 1-5

[16] Philips N, Tahir M, Stellatella J, Stephan K, Givant J, Zhou L, Costa A, Taw M (2009) Differential Regulation of Growth Factors and Matrix Metalloproteinase-1 by Estrogen, Progesterone, and Tamoxifen in Normal and Cancerous Endometrial cells. J. Cancer Mol. 4: 169-173.

[17] Philips N, Tuason M, Chang T, Lin Y, Tahir M, Rodriguez SG (2009) Differential effects of Ceramide on Cell Viability and Extracellular Matrix Remodeling in Keratinocytes and Fibroblasts. Skin Parmacol. Physiol. 22:151-157.

[18] Philips N, Conte J, Chen Y, Natrajan P, Taw M, Keller T, Givant J, Tuason M, Dulaj L, Leonardi D, Gonzalez S (2009) Beneficial Regulation of Matrixmetalloproteinases and its Inhibitors, Fibrillar Collagens and Transforming Growth Factor-b by P. leucotomos, Directly or in Dermal Fibroblasts, Ultraviolet Radiated Fibroblasts, and Melanoma Cells. Arc. Derm. Res.: 301:487-495

[19] Philips N, Dulaj L, Upadhya T. (2009) Growth inhibitory mechanism of ascorbate and counteraction of its Matrix Metalloproteinases-1 and Transforming Growth Factor-beta stimulation by gene silencing or P. leucotomos AntiCancer Research in press.

[20] Philips N, Arena R, Chen Y, Conte J, Natrajan P, Haas G, Gonzalez S. (2009) Direct Inhibition of Elastase and Matrixmetalloproteinases, and Stimulation of Biosynthesis of Fibrillar Collagens, Elastin and Fibrillins by Xanthohumol. J Cosmet. Sci. in press

[21] Philips N, Hwang H, Chauhan S, Leonardi D, Haas G, Gonzalez S. (2009) Stimulation of cell proliferation, and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper. Connective Tissue Research: in press

Book Chapters

[1] Philips N. (2009) Reciprocal Effects of Ascorbate on Cancer Cell Growth and the Expression of Matrix metalloproteinases and Transforming Growth Factor-beta: Modulation by Gene Silencing or P. leucotomos. Hanbook of Vitamin C Research: Daily Requirements, Dietary Sources, and Adverse Effects. Editors: Hubert Kucharski and Julek. Nova Science Publishers, Inc. ISBN: 978-1-60741-874-0

[2] Philips N. (2009) Experimental Physiology in Anti-Skin Aging. Skin Anatomy and Physiology Research Development. Editor: Frank Columbus. Nova Science Publishers, Inc.

Review Papers

[1] Philips N. (1997) Altered expression of collagen and elastic fibers in photodamaged skin. Dermatol Cosmet 2: 129-130.

[2] Philips N. (1998) Molecular effects of benzene/benzene metabolites on skin cells. Dermatol Cosmet 8: 97-100.

[3] Resetar JM. and Philips N. (1998) Clinical pathology and epidemiology of

the Ebola virus: investigating immune response. Dermatol Cosmet 8: 113-118.

[4] Koskowicz K. and Philips N. (1999) Extracellular matrix proteins and its effects on fibroblasts and keratinocytes. Dermatol Cosmet 9: 95-101.

[5] Philips N. and Newman J. (2000) Correlations of cellular senescence with alterations in immune response, and expression of transforming growth factor-beta (TGF-b) and interstitial collagenase. Dermatol Cosmet 10: 15-17.

[6] Editor’s report, (2007). Focus on research. The fern P. leucotomos-From ethnomedicine to documented photoimmunoprotection. Dermatology Focus 25: 16-18

[7] Philips N (2008). Experimental Biochemistry on Skin Anti-Aging. Cosmetiscope 14 (2): 4

[8] Bautista M, Lockett F, Mecca J, Sawatdeekhachornphat W, Castro A, Yarlagadda S, Gonzales S, and Philips N. (2008) The use of antisense oligonucleotides in skin lightening products. J of Plast Dermatol 4: 17-19.

[9] Goodner J, Patil U, Lim Y, Yarlagadda S, Castro A, Gonzales S, and Philips N. (2008) Coating on micronized titanium dioxide increases safety and maintains efficacy as sunscreen filter. J of Plast Dermatol 4: 21-22.

II. Grant proposals/Funding and Reports

I. Industrial Farmaceutica Cantabria (IFC) S.A., 28043 Madrid, C/ Arequipa, Spain.

(1) Regulation of Extracellular Matrix, Heat Shock Proteins, and Transforming Growth Factor-b (TGF-b) by Polypodium leucotomos (PL) in Preventing Skin Aging (January 2007 − December 2008).

(2) Molecule 168 and Derivatives: Regulation of Extracellular Matrix and Heat Shock Proteins (May 2008 − December 2008).

(3) Improvement of Epidermal Barrier Function by Polypodium leucotomos in Preventing Skin Photo-aging (May 2008 − December 2009).

(4) Anti-inflammatory and Antiangiogenic Mechanism of Polypodium leucotomos (May 2008 − December 2009).

II. Research Release time, University College, Fairleigh Dickinson University, NJ.

(1) Regulation of Extracellular Matrix, Heat Shock Proteins, and Transforming Growth Factor-b (TGF-b) by Polypodium leucotomos (PL) in Preventing Skin Aging (Six credits) (September 2007 − May 2008).

(2) Improvement of Epidermal Barrier Function and Prevention of Tumorigenesis by Polypodium leucotomos, via Anti-inflammatory and Anti-angiogenic Mechanisms (Three credits) (January 2010 - May 2010)

III. Grant-in-Aid program, University College, Fairleigh Dickinson University, NJ.

(1) Investigation of Cancer Mechanisms in an Ascorbate Model: Regulation by Lutein and Specific Gene Silencing (June 2007 − May 2008)

(2) Investigation and Management of Ascorbate Effects in Cancer Treatment (June 2008 − May 2009).

(3) Inhibition of Angiogenesis and Immune Cell Preservation in Cancer Management by Ascorbate and P. leucotomos (July 2009 − June 2010).

IV SEED grant, Fairleigh Dickinson University, NJ.

(1) Elastin Gene Silencing in Keratinocytes for the Management of Solar Skin Aging (September 2007 − August 2008).

Reports

[1] Philips N. (2008). Inhibition of Matrixmetalloproteinases, and Stimulation of Tissue Inhibitors of Matrixmetalloproteinases, Transforming Growth Factor-b, Fibrillar Collagens, and Heat Shock Proteins by P. leucotomos and Anti-Skin Aging

[2] Philips N. (2008). Stimulation of Tissue Inhibitors of Matrixmetalloproteinases, Fibrillins and Survival in Keratinocytes by P. leucotomos

[3] Philips N. (2008). Direct Inhibition of Matrixmetalloproteinases and elastase; Stimulation of fibrillar collagens, elastin, fibrillins and heat shock proteins in non-irradiated and UVA radiated fibroblasts; and Stimulation of type III collagen, Elastin and Fibrillins in UVB radiated Fibroblasts by SE 1856

[4] Philips N. (2008). SE 1857 Effects: Direct Inhibition of Matrixmetalloproteinases and elastase; and Cellular Protection to Non-Irradiated and UVA-Irradiated Fibroblasts via Stimulation of types I, III, and V collagen; Elastin and Fibrillins; and Heat Shock Proteins-27, 47, 70, 90

[5] Philips N. (2008). Direct Inhibition of Matrixmetalloproteinases and elastase and Stimulation of fibrillar collagens (types I, III, V); Elastin, Fibrillin-1 and Fibrillin-2; and Heat Shock Proteins (27, 47, 70 and/or 90) in Non-irradiated, UVA-radiated, and UVB radiated Fibroblasts by SE 1858

[6] Philips N. (2009). Stimulation of fibrillar collagens (types I, III, V); Elastin, Fibrillin-1 and Fibrillin-2; and Heat Shock Proteins (27, 47, 70 and/or 90) in Non-irradiated, UVA-radiated, and UVB radiated Fibroblasts by SE 1910

III. Reasearch Methods and Equipment

Research Methodology:

(1) Cell and tissue culture, Maintenance, and Experimentation in cells or tissue-equivalents; Skin (fibroblasts, keratinocytes, skin equivalents) and cancer (melanoma, renal, mammary, endometrial);

(2) Cell viability, Proliferation, Toxicity and Apoptosis;

(3) Recombinant DNA Technology; Polymerase chain reaction (PCR), Cloning, Site directed mutagenesis;

(4) Transcriptional Regulation: Transfection of promoter-reporter plasmids and

reporter (chloramphenicol acetyl transferase, luciferase, beta-galactosidase) assays;
Promoter/protein interactions (Gel shift and super shift assays); 
(5) mRNA analysis: RNA isolation, Northern blot hybridization, RNase protection, RT- 
PCR, Real time PCR, RNA interference. 
(6) Protein analysis: ELISA, Zymograms, Western blotting, Immunoprecipitation;

(7) Oxidative stress: Assays for Reactive oxygen or nitrogen species, Lipid peroxidation

and Antioxidant assays;

(8) Enzyme kinetics and Enzyme activity Inhibition: Matrix metalloproteinases and

Elastase;

(9) Histology: Paraffin embedding, Cryostat, Slide preparation, H&E staining,

Immunohistochemistry, Immunoflurescence.

Instruments: Tissue culture Hoods (Baker, Herasafe); Microplate Readers/data analysis - absorbance, luminescence and fluorescence (Berthold Mithras LB490; Biotek Synergy HT; Dynex); Electrophoresis (Amersham Multiphor II system, Bio Rad system; Invitrogen e-PAGE); Imaging (Imgen technologies, alpha ease program); iCycler iQ (Bio Rad); Microscopes (Inverted; Leica CTR 6000 with fluorescence imaging); Histology (Leica- Microtom, cryostat, paraffin embedding center).

IV. Presentations/Abstracts

Presentations

[1] Philips N, Bashey RI, Donnelly M and Jimenez SA. (1991)

Increased collagen gene expression in cultured fibroblast-like cells from hypertrophic myocardium Faseb J 5:A1245 (Oral: Annual meeting FASEB, Georgia)

[2] Philips N and Blumemberg M. (1998) Regulation of expression of elastin, collagenase and type I collagen in keratinocytes, fibroblasts and co-cultures of keratinocytes and fibroblasts. Bull NJ Acad Sci 43 (Oral: Annual meeting New Jersey Academy of Science, New Jersey)

[3] Philips N, Burchill D, O’Donoghue D, Gonzalez S. (2001) Dermal fibroblasts convert benzene to non-phenolic compounds that are less toxic than the phenolic metabolites of benzene. J Invest Dermatol 117: 481 (Poster: Society of Invest. Dermatol., Washington DC)

[4] Philips N and Devaney J. (2001) Hormone replacement therapy increases type I collagen in dermal fibroblasts by stimulating transforming growth factor-beta. J Am Aging 24: 129 (Poster: Annual meeting of AGE/American College of Clinical Gerontology/AFAR).

[5] Philips N, Devaney J, Smith J, Hamilton S, Gonzalez S. (2002) Hormones, Polypodium leucotomos, and lutein, respectively, regulate expression of extracellular matrix proteins in skin fibroblasts. Biogerontology 3: 93 (Oral: European Congress of Biogererontology, Italy)

[6] Philips N. (2003) Skin anti-aging and anti-carcinogenic effects of lutein, and Polypodium leucotomos. J Am Aging (Oral: American Aging Association, Maryland)

[7] Philips N, Keller T, Smith J, Gonzalez S. (2003) Regulation of matrix metalloproteinases (MMPs), and tissue inhibitors of matrix metalloproteinases (TIMPs) by anti transforming growth factor-b antibodies, lutein and Polypodium leucotomos. Mol. Cell. Proteonomics: 2: 923 (Oral: HUPO Annual and IUBMB World Congress, Montreal, Canada)

[8] Philips N. (2005) Skin Firming activity of Rhodiola via induction of elastin;

Cofactors for increased skin metabolic activity (Oral: Web conference, Chanel, NJ)

[9] Philips N. (2006) The Cellular and Molecular Biology of Aging and Cancer (FDU Seminar).

[10] Philips N (2007) Differential Dose Dependent Effects of Ascorbate on Cancer Cell Toxicity or Proliferation by Ascorbate; Regulation of Matrix metalloproteinases and Transforming Growth Factor-beta. Expt. Biol. LB98 (Poster: ASBMB, Washington DC).

[11] Philips N (2007) Regulation of the Extracellular Matrix Remodeling by Lutein in Dermal Fibroblasts, Melanoma cells, and Ultraviolet Radiation Exposed Fibroblasts. Cancer Drug Discovery (Poster: Drug Discovery and Development of Innovative Therapeutics, Boston)

[12] Philips N. (2007) Semester-Long Application-Based Investigative Project, in Modern Cell Biology, for Broader Learning Outcomes Session (Poster: ASCB, Washington DC)

[13] Philips N. (2008). Experimental Biochemistry in Anti-Skin Aging (Oral: SCC, NJ)

[14] Philips N. (2008) Anti: Skin Aging and Cancer (FDU Seminar).

[15] Philips N. (2008) Photoaging, and Cancer Treatment by Gene Silencing or Micronutrients (Poster: Drug Discovery and Development of Innovative Therapeutics, Boston)

[16] Philips N. (2009) Inflammation; Immunity:Antibodies; Signaling Pathways that Control Gene Activity (Oral: Presperse Inc, Somerset, NJ)

[17] Philips N. (2009) Aging, Wound Healing and Cancer (FDU Seminar).

Research Students Presentations/Abstracts (74)

A. Ultraviolet radiation, oxidants or/and anti-oxidants

Polypodium leucotomos, lutein or flavanoid (40 abstracts)

Skin (fibroblasts, keratinocytes)

[1] Newman J and Philips N. (1999) Molecular correlation of ICAM-1

(intercellular adhesion molecule-1) and cellular senescence Bull NJ Acad Sci 44

[2] Clark T and Philips N. (1999) Effect of nitric oxide on ultraviolet irradiated keratinocytes. Bull NJ Acad Sci 44

[3] Golovaty E and Philips N. (1999) Say no (nitric oxide) to hydrogen peroxide

cytotoxicity in dermal keratinocytes. Bull NJ Acad Sci 44

[4] Newman J and Philips N. (2000) Activity and secretion of ICAM-1 by oxidants, antioxidants and combinations of oxidants and antioxidants, in dermal fibroblasts. Bull NJ Acad Sci 45

[5] Avramidis A and Philips N. (2000) Regulation of extra cellular matrix

(ECM) proteins, growth factors and intercellular adhesion molecule-1

(ICAM-1) expressions by nitric oxide (NO) in dermal fibroblasts Bull NJ

Acad Sci 45

[6] Clark T and Philips N. (2000) Effects of ultraviolet radiation and nitric oxide

(NO) in dermal fibroblasts. Bull NJ Acad Sci 45

[7] Avramidis A and Philips N (2001) Regulation of extra cellular matrix (ECM) proteins, growth factors and intercellular adhesion molecule-1 (ICAM-1) expressions by nitric oxide (NO) in dermal fibroblasts Bull NJ Acad Sci 46

[8] Parratt A and Philips N. (2001) Potentiation of ultraviolet induced generation of hydrogen peroxide by TGF-b, hydrogen peroxide and a nitric oxide scavenger. Bull NJ Acad Sci 46

[9] Smith J, Gonzalez S and Philips N. (2001) Antagonistic effects of ultraviolet

radiation and Polypodium leucotomos on skin keratinocytes and fibroblasts.

Bull NJ Acad Sci 46

[10] Parratt A and Philips N. (2002) Induction of elastin expression by ultraviolet radiation in human dermal keratinocytes (Sigma Xi research symposium)

[11] Cassidy S, Karl S, Myatt S and Philips N. (2003) Anti-aging effects of mild stress on fibroblast cells. Bull NJ Acad Sci 47

[12] Covert S, David DG and Philips N. (2003) Xanthophyll and its role in aging

Bull NJ Acad Sci 47

[13] Hamilton S and Philips N. (2002) Effects of lutein on skin aging (Sigma Xi)

[14] Hendrix C and Philips N. (2003) Protective Effect of lutein on skin aging (Sigma Xi research symposium)

[15] Ekelhar A, Surgent B and Philips N. (2003) Dose dependent responses of early and late passage fibroblasts to Polypodium leucotomos extract. Bull NJ Acad Sci 47

[16] Woo J and Philips N. (2007) Increased Expression of elastin in ultraviolet radiation exposed keratinocytes (Hudson-Bergen Chemical Society, SJU Sigma Xi Research Symposium)

[17] Natarajan P and Philips N. (2008) Mechanisms of skin regeneration by a mollusk secretion (Hudson-Bergen Chemical Society, FDU Bio-Seminar).

[18] Thota S, Yarlagadda S and Philips N. (2008) Large scale plasmid preparation and promoter activity testing (Hudson-Bergen Chemical Society).

[19] Yarlagadda S and Philips N. (2008) Anti skin aging effect of luteolin (Hudson-Bergen Chemical Society).

[20] Conte J, Scorfield J and Philips N. (2008) Screening activities in elastase enzyme inhibition assays (Hudson-Bergen Chemical Society).

[21] Plaskon P, Pedrick M, Fields T and Philips N. (2009) Epidermal protective effects of vitamin D (Hudson-Bergen Chemical Society).

[22] Baswan, S and Philips N. (2009) Anti-Skin aging potential of xanthohumol by

stimulation of fibrillin expression (Hudson-Bergen Chemical Society)

[23] Chauhan, S and Philips N. (2009) Regulation of heat shock proteins by p. leucotomos and its components in skin cells (Hudson-Bergen American Chemical Society).

[24] Dodiya, R and Philips N. (2009) Polyphenols stimulate structural collagens (Hudson-Bergen Chemical Society, Biology Seminar, FDU Biology Seminar).

[25] Jain, D and Philips N. (2009) Dual protective effects of p. leucotomos and its components on elastic fibers (Hudson-Bergen Chemical Society, FDU Biology Seminar).

[26] Perez E, Kogan A, Yamada E and Philips N. (2009) regulation of apoptosis for cell survival by P. leucotomos in ultraviolet radiation exposed keratinocytes (Hudson-Bergen Chemical Society).

[27] Sharma K, Nicolas C, Baswan S and Philips N. (2009) Regulation of collagen,

elastin and heat shock proteins by caffeic acid (Hudson-Bergen Chemical Society, Society of Cosmetic Chemists Annual Meeting).

[28] Tuason, M and Philips N. (2009) Beneficial remodeling of the extracellular matrix by P. leucotomos and its components (Hudson-Bergen American Chemical Society).

Lung (fibroblasts)

[29] Prashanthi N and Philips N. (2007) Regulation of cell viability and matrixmetalloproteinases in lung fibroblasts by Xanthohumol (Hudson-Bergen Chemical Society, National Collegiate Honors Conference, FDU Bio-Seminar)

[30] Schmidt E, Lescisko A and Philips N. (2007) Oxidative effects of hydrogen peroxide in lung (MRC5) fibroblasts (Hudson-Bergen Chemical Society, SJU Sigma Xi Research Symposium)

Cancer (melanoma, renal, mammary or endometrial cancer, vero)

[31]Rizzo L and Philips N. (1999) Antioxidants affect the growth and collagenase expression of mammary epithelial cells. Bull NJ Acad Sci 44

[32] Stephan K, Harpe A, Leonard C and Philips N. (1999) Nitric oxide and its effects on 10A, 10F, and MCF-7 cells. Bull NJ Acad Sci 44

[33] Carey J and Philips N. (2001) Effects of oxidants and antioxidants on renal adenocarcinoma cell proliferation and metastasis. Bull NJ Acad Sci 46

[34] Massa T, Vaccaro S, Vestyck L and Philips N. (2003) Regulation of matrixmetalloproteinases (MMP) in melanoma cells by hydrogen peroxide Bull NJ Acad Sci 47

[35] Matlosz T, Patel P, DeGregorio D and Philips N. (2003) Differential effects of hydrogen peroxide on MMP-1, MMP-2, and TGF-β mRNA levels in

renal cancer cells Bull NJ Acad Sci 47

[36] Gocek D, Wines P and Philips N. (2003) Anti-carcinogenic effects of

xanthophyll. Bull NJ Acad Sci 47

[37] Inzano CA, Ord L and Philips N. (2003) Dose responsive effects of

Polypodium leucotomos on MMP-1, MMP-2 and TGF-b in renal and melanoma cells. Bull NJ Acad Sci 47

[38] Arena R and Philips N. (2007) Identification of anti-carcinogenic agents in the treatment of melanoma (Hudson-Bergen Chemical Society, SJU Sigma Xi Research Symposium, FDU Bio-Seminar)

[39] Pitropaki A, Aviles D and Philips N. (2007) Selective inhibition of melanoma cell viability by Polypodium leucotomos (SJU Sigma Xi Research Symposium)

[40] Kogan A, Padilla S and Philips N. (2008) Molecular mechanisms of luteolin’s anticarcinogenic effect (Hudson-Bergen Chemical Society, FDU Bio-Seminar).

B. TGF-β or anti-TGF-b antibodies (8 abstracts)

Skin (fibroblasts, keratinocytes)

[1] Novak K and Philips N. (2000) Effects of anti-TGF-b antibodies on TGF- b secretion, extracellular (ECM) proteins and DNA apoptosis in dermal fibroblasts. Bull NJ Acad Sci 45

[2] Sodt R and Philips N. (2000) Effects of dioxins and anti TGF antibodies on

oxidative stress response in dermal fibroblasts. Bull NJ Acad Sci 45

[3] Keller T and Philips N. (2002) Regulation of matrix metalloproteinases by anti

transforming growth factor beta in fibroblast cells (Sigma Xi research symposium)

[4] Keller T and Philips N. (2003) Regulation of matrix metalloproteinases by

anti transforming growth factor-beta antibodies in dermal fibroblasts. Bull

NJ Acad Sci 47

Lung (fibroblasts)

[5] Goldschein E and Philips N. (2007) Regulation of tissue inhibitor of matrixmetalloproteinases by transforming growth factor- beta: Modulation by ultraviolet radiation (Hudson-Bergen American Chemical Society, SJU Sigma Xi Research Symposium, FDU Bio-Seminar)

Cancer (melanoma, renal, mammary or endometrial cancer, vero)

[6] Onwubalili N, and Philips N. (1999) Proliferative effect of the TGF-

antibodies; anti-TGF- β and anti-LAP, on immortalized human mammary

epithelial (MCF-10A) cells. Bull NJ Acad Sci 44.

[7] Onwubalili N. and Philips N. (2000) Proliferative effect of the TGF-β antibodies, anti-TGF-β and anti-LAP, on renal adenocarcinoma cells (Tri-beta meeting presentation)

[8] Monteleone J, and Philips N. (2002) Increased cell proliferation and expression of

transforming growth factor alpha and matrix metalloproteinase–2 by anti

transforming growth factor beta (Sigma Xi research symposium)

C. Ascorbic acid (6 abstracts)

Cancer (melanoma, renal, mammary or endometrial cancer, vero)

[1] Slavick S and Philips N. (2000) Differential effects on hydrogen peroxide and

ascorbic acid on renal adenocarcinoma and uterine endometrial cells. Bull NJ

Acad Sci 45.

[2] Carey J and Philips N (2002) Regulation of proliferation and expression of growth

factors, and matrix metalloproteinases in renal, skin, and mammary cancer

cells (Sigma Xi research symposium)

[3] Hourmouzis P, Plaskon P, McKelvey K, Avendano A and Philips N. (2007) Ascorbate in combination with Vitamin E in cancer treatment (Hudson-Bergen Chemical Society, SJU Sigma Xi Research Symposium)

[4] Semilla A and Philips N. (2008) Gene silencing for photoaging and cancer therapy (Hudson-Bergen Chemical Society, FDU Bio-Seminar).

[5] Givant J and Philips N. (2008) Mechanisms regulating differential regulation of MMP1 by ascorbate (Hudson-Bergen Chemical Society, NJ Acad. Sci., Junior academy).

[6] Dulaj, L and Philips N. (2009) Combination of Vitamin C and P. leucotomos is Beneficial to Cancer Therapy (Hudson-Bergen Chemical Society, FDU Biology Seminar).

D. Ceramide (3 abstracts)

Skin (fibroblasts, keratinocytes)

[1] Hanrahan S and Philips N. (1999) The role of ceramide (n-acetyl d-sphingosine) in the regulation of extracellular matrix proteins in fibroblasts. Bull NJ Acad Sci 44

[2] Hamilton S and Philips N. (2001) Transcriptional regulation of oxidative stress and collagenase genes by ceramide in keratinocytes. Bull NJ Acad Sci 46

[3] Lin Y, Plaskon P, Chang T and Philips N. (2008) Epidermal differentiation mechanism of ceramide (Hudson-Bergen American Chemical Society, FDU Bio-Seminar).

E. Benzene or benzene metabolites, Copper (6 abstracts)

Skin (fibroblasts, keratinocytes)

[1] Burchill D and Philips N. (1999) Benzene metabolism by human skin cells.

Bull NJ Acad Sci 44

[2] O’Donoghue D and Philips N. (1999) Effect of benzene metabolites on the toxicity and apoptosis of human dermal fibroblasts. Bull NJ Acad Sci 44

[3] Rosenblatt J, Parratt A, Burchill D and Philips N. (2000) Nontoxic effects of benzene metabolites on human dermal fibroblasts. Bull NJ Acad Sci 45

[4] Ryan J and Philips N. (2001) Oxidative and aging effects of benzoic acid on dermal fibroblasts. Bull NJ Acad Sci 46

[5] Ryan J and Philips N. (2002) Oxidative and aging effects of benzoic acid on dermal

fibroblasts (Sigma Xi research symposium)

[6] Kahodariya J, Mehta N and Philips N. (2008) Role of copper in wound healing (Hudson-Bergen Chemical Society, FDU Bio-Seminar).

F. Hormones (11 abstracts)

Skin (fibroblasts, keratinocytes)

[1] McGovern A and Philips N. (1999) The effect of estrogen on the promoter activity and expression of collagenase in dermal keratinocyte, MCF-7 and MCF- 10F cells. Bull NJ Acad Sci 44

[2] Nelson M, Lasko M, Loder C and Philips N. (1999) Effects of estrogen treatment and varied matrix substrates on secretion of elastin, collagenase, and TGF-b by fibroblasts, keratinocytes, and co-cultures of fibroblasts and keratinocytes. Bull NJ Acad Sci 44

[3] Devaney J and Philips N. (2001) Hormone replacement therapy increases collagen expression in human dermal fibroblasts via release of active TGF-β. Bull NJ Acad Sci 46.

Cancer (melanoma, renal, mammary or endometrial cancer, vero)

[4] McFadden K and Philips N. (1999) Effect of prolactin on the secretion of extracellular matrix proteins in immortalized and neoplastic mammary cells. Bull NJ Acad Sci 44

[5] McFadden K and Philips N. (2000) The effect of the female sex hormones prolactin and estrogen on the secretion of MMP1 and TGF-b in both normal and turmorigenic mammary epithelial cells. Bull NJ Acad Sci 45

[6] Vorselen H and Philips N. (2000) The response of mammary cancer cells to treatment with tamoxifen. Bull NJ Acad Sci 45

[7] Stellatella J and Philips N. (2000) Effects of hormone treatment on cell proliferation and production of growth factors and collagenase in normal and carcinogenic endometrial cells. Bull NJ Acad Sci 45

[8] Stephan K and Philips N. (2000) Regulation of interstitial collagenase promoter activity by tamoxifen in normal uterine endometrial cells. Bull NJ Acad Sci 45

[9] Hurley C and Philips N. (2001) Beneficial effects of hormone replacement therapy on endometrial cells. Bull NJ Acad Sci 46

[10] Monteleone J and Philips N. (2001) Combination of estrogen and prolactin increases matrix

metalloproteinase-2 by inhibiting TGF-b in mammary epithelial cells. Bull NJ Acad Sci 46

[11] Lee S and Philips N. (2008) Tamoxifen’s effectiveness in endometrial cells (Hudson-Bergen Chemical Society).

V. Courses Taught. UG: Undergraduate. GR: Graduate

Cell Biology(lecture and laboratory) (UG): Modern Cell Biology investigates the central concept for cellular functioning, DNA to RNA to Protein, by integrating biomolecules, biotechnology, regulating mechanisms, signals and signaling pathways and aberrations that result in pathology, such as cancer. Application of cell/molecular biology methodology to a research project: to examine regulation of cell viability/proliferation, toxicity, proteins, mRNA and gene activity via guided experiments for each regulatory level as well as independent experimental planning and performance. Reinforcement of concepts/applications through problem-solve tests and industry style reports, and comprehensive synthesis/communication in the format of a journal article and an oral presentation.

Biochemistry I and II (lecture and laboratory) and Topics in Biochemistry (lecture) (UG): Structure and function of DNA, RNA and proteins, recombinant DNA technology, metabolic pathways and hormonal regulation, signal transduction and membrane dynamics, antibodies, DNA replication and repair, RNA synthesis and modifications, protein synthesis and targeting, prokaryotic and eukaryotic gene expression and regulation. The Topics in Biochemistry course corresponded to research and pharmaceutical literature in Biochemistry.

Techniques: Theories and Applications (lecture and laboratory) (UG): The goal of the course is teach essential fundamental, advanced and current techniques in chemistry/biochemistry and molecular biology. These techniques include handling numbers; weighing and solution preparation; experiment designing and planning technical process; spectrophotometry; chromatography; enzyme kinetics/inhibition; assays for reactive oxygen species; cell culture, hemocytometer and microscopes; agarose and polyacrylamide gel electrophoresis; Southern and Western blotting blotting; cloning, plasmid preparation and expression vectors in eukaryotic cells; polymerase chain reaction (PCR) and real time-PCR; and discussion on nucleic acid arrays.

General BiologyI (lecture) (UG): encompasses two areas of studies: the diverse organisms, their organization/classification, and evolutionary history/relationship; and the architecture and functioning of organ systems that maintain body’s homeostasis.

General BiologyII (lecture) (UG): General Biology II is the foundation to cell and molecular biology. It is comprised of Molecules and Cells; Genetics; and Evolutionary Biology. It is focused on fundamental biological concepts, their development, research and scientific process.

Comprehensive synthesize and presentation of an area of the lecture, an associated methodology, and application to real life biological process - using lecture information, research articles and web-sites’ information

General BiologyII (laboratory) (UG): General Biology II laboratory is the hands-on counterpart to General Biology II lecture. The emphasis is on the scientific process / hypothesis testing, and scientific communication as abstract, introduction, methods, results, discussion and literature cited. It is comprised of experiments in Molecules and Cells; Genetics; and Evolutionary Biology.

Modern Biology (lecture) (UG): Atoms, Molecules, and Cells, Genetics, Animal Anatomy and Physiology, Energy and its Transformations, and Evolution and the Diversity of Life. Role of science in everyday life; Scientific Method; Scientific communication

Integrated Science I and II (lecture and laboratory) (UG); Integrated Science: Incorporation of the principles in biology, chemistry, physics and earth science. Topics include matter, energy, entropy, thermodynamics, electricity, magnetism, atomic and nuclear structure, chemical principles, chemical reactions, organic chemistry, biochemistry, rocks, planets, genetics, ecology, and evolution. Laboratory experiences demonstrate the principles presented in lecture. 

Advanced Cell Biology (lecture and laboratory) (GR): Cell structure and function, signals, receptors and signal transduction, recombinant DNA technology and its application in biotechnology, transcription initiation and regulation, mRNA processing, gene expression and regulation, nerve cell physiology and pharmacology, cell cycle regulation and cancer

Tissue Culture(lecture and laboratory): Tissue culture applications in genetics, gene expression, intracellular activity, and secretion of cell products, cell-cell and cell-environment interactions, and tumorigenesis. Cell culture biology, primary culture, cell propagation, maintenance, and cryopreservation; quantitation and cytotoxicity; primary cell culture and cell cloning; cell differentiation and transformation; molecular techniques 

Microtoxicity and Biochemistry (lecture) (GR): The underlying microtoxicity/biochemistry principles in cosmetic skin care: Fundamental concepts of bio-molecules (structure, function, expression, regulation); metabolic pathways and their integration; skin structure, inflammation, aging and diseases; active ingredients in skin cleansers and moisturizers; delivery of cosmetic products. Delineation of the microtoxicity/biochemistry of current skin care procedures, biological targets and new technology

Recombinant DNA technology (lecture) (GR): It is fundamental to molecular biotechnology that encompasses many scientific disciplines (i.e. molecular biology, microbiology, biochemistry, immunology, genetics, chemical engineering, cell biology) and generates a wide range of consumer products (i.e. crops, drugs, vaccines, diagnostics, and livestock). Topics covered are text (in syllabus, section II) chapters : “Molecular Biotechnology Biological Systems, Gene Regulation, Recombinant DNA Technology, Chemical Synthesis, Sequencing, and Amplification of DNA, Manipulation of Gene Expression in Prokaryotes, Heterologous Protein Production in Eukaryotic cells, Directed Mutagenesis and Protein Engineering, Molecular Diagnostics, Therapeutic Agents, Vaccines, Synthesis of Commercial Products by Recombinant Microorganisms, Large-Scale Production of Proteins from Recombinant Microorganisms, Transgenic Animals, Regulating Use of Biotechnology, Patenting Biotechnology Inventions”

Biotechniques (lecture and laboratory) (GR): Current biotechnology techniques, including DNA cloning, gene regulation and protein expression/activity, using in-vitro models as wells as prokaryotic, and eukaryotic cells or tissue-equivalents. The laboratory is the hands-on counter-part to lecture: Molecular biotechniques of wide applicability; Different biological systems used in molecular biotechnology; DNA cloning, plasmid engineering, plasmid selection and propagation; Site-directed mutagenesis, polymerase chain reaction, selection of mutants; Prokaryotic expression of protein, protein isolation and analysis by SDS-PAGE and/or Western blotting; Eukaryotic transformation of a wild type or mutant gene, genomic organization of stable transformants, and cellular localization of expressed protein; Notebook records of experiments, presentations, group discussions, manuscript preparation, and creation of a proposal for an independent experiment, via accessing and adapting a molecular technique to problem solve a research question

Genetics Workshop (high school advanced placement biology students): Restriction enzyme digestion of DNA, gel electrophoresis, polymerase chain reaction, and transformation to examine gene expression.

VI. Scientific Community Service

Reviewer: Manuscripts, Textbooks, Proposals

Manuscripts:

Skin Pharmacology and Physiology (S. KARGER AG, BASEL),

Experimental Dermatology (Blackwell Publishing)

BBA-Molecular Cell Research (Elsevier)

British Journal of Cancer (Blackwell Publishing

Ecotoxicology and Environmental Safety (Elsevier

Current Drug Targets (Bentham Science Publishers)

Dermatology Research and Practice (Hindawi Publishers)

Clinical, Cosmetic and Investigational Dermatology (Dove Medical Press)

The Open Biotechnology Journal (Bentham Science Publishers)

Text Books: Academic Press/Elsevier

Go-Tutorials: Wiley

Grant Proposal: Innovational Research Incentive Scheme (Netherland Organization for Scientific Research)

Consultant or Collaborator: Presperse, Somerset, NJ; Bergen Academies, Hackensack, NJ